Journal: Redox biology
Article Title: Lipoxin A 4 ameliorates knee osteoarthritis progression in rats by antagonizing ferroptosis through activation of the ESR2/LPAR3/Nrf2 axis in synovial fibroblast-like synoviocytes.
doi: 10.1016/j.redox.2024.103143
Figure Lengend Snippet: Fig. 2. Downregulation of ESR2, LPAR3, and GPX4 and upregulation of HO-1 and CGRP levels in synovial tissue with increasing Kellgren–Lawrence (KL) grades. (A) Immunohistochemical staining results for ESR2, LPAR3, GPX4, HO-1, and CGRP. (B–F) Statistical analysis of the immunohistochemical staining results. A comparison of all KL grades with grade 0 is shown (*P < 0.001, *aP = 0.004, *bP = 0.002, *cP = 0.039, *dP = 0.03, *eP = 0.009, ANOVA). Data are expressed as the mean ± 95% confidence interval; n = 3 patients per group.
Article Snippet: Then, the following primary antibodies were added: rabbit polyclonal anti-ESR2 antibody (14007-1-AP, 1:1000; Proteintech), rabbit polyclonal anti-LPAR3 antibody (orb394997, 1:1000; Biorbyt), rabbit monoclonal anti–HO–1 antibody (ab189491, 1:2000; Abcam), rabbit monoclonal anti-GPX4 antibody (ab125066, 1:500; Abcam), rabbit monoclonal anti-SOD1 antibody (ab308181, 1:100; Abcam), rabbit polyclonal anti-CGRP antibody (16630-1-AP, 1:100; Proteintech), overnight at 4 ◦C in a cold room.
Techniques: Immunohistochemical staining, Staining, Comparison